Journal: Current Biology

Article Title: Ventro-dorsal Hippocampal Pathway Gates Novelty-Induced Contextual Memory Formation

doi: 10.1016/j.cub.2020.09.074

Figure Lengend Snippet:

Article Snippet: Mouse anti-RFP IgG1k , MBL , Cat# M165-3; RRID: AB_1520843.

Techniques: Virus, Plasmid Preparation, Recombinant, RNAscope, Multiplex Assay, Software

Journal: Communications Biology

Article Title: A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplification

doi: 10.1038/s42003-025-08317-z

Figure Lengend Snippet: a Schematic diagram of an experimental procedure for testing tissue penetration of POD-nAbs and conventional Abs. b –e Tissue penetration of a POD-nAb ( b ) and conventional Abs against GFP ( c –e ). 1-mm-thick brain slices infected with AAV2/PHP.eB CAG-EGFP-WPRE are reacted with a POD-nAb (GFP1 POD-nAb) ( b ), chicken (Ch) polyclonal Ab ( c ), rabbit (Rb) polyclonal Ab ( d ), and rat (Rt) monoclonal Ab ( e ) against GFP. b 1 , c 1 , d 1 , e 1 Representative images of immunoreactivity (IR) for GFP Abs in the cerebral cortex. b 2 , c 2 , d 2 , e 2 Merged images of IR for GFP (magenta) and EGFP fluorescence (green) in the cerebral cortex. f –i Tissue penetration of a POD-nAb ( f ) and conventional Abs against RFP ( g –i ). 1-mm-thick brain slices infected with AAV2/PHP.eB CAG-tdTomato-WPRE are reacted with a POD-nAb (RFP6 POD-nAb) ( f ), guinea pig (Gp) polyclonal Ab ( g ), goat (Gt) polyclonal Ab ( h ), and mouse (Ms) monoclonal Ab ( i ) against RFP. f 1 , g 1 , h 1 , i 1 Representative images of IR for RFP Abs in the cerebral cortex. f 2 , g 2 , h 2 , i 2 Merged images of IR for RFP (magenta) and tdTomato fluorescence (green) in the cerebral cortex. j, k Histograms showing immunoreactive intensity ratio between the center ( ≥ 400 µm from the surface) and the periphery ( ≤ 100 µm from the surface) of brain slices stained with GFP ( j ) and RFP ( k ) Abs. The intensity value is normalized by EGFP or tdTomato fluorescence ( n = 15 sections from 3 animals, GFP1 POD-nAb; n = 15 sections from 3 animals, Ch GFP Ab; n = 14 sections from 3 animals, Rb GFP Ab; n = 14 sections from 3 animals, Rt GFP Ab; n = 20 sections from 4 animals, RFP6 POD-nAb; n = 15 sections from 3 animals, Gp RFP Ab; n = 20 sections from 4 animals, Gt RFP Ab; n = 14 sections from 3 animals, Ms RFP Ab; GFP Abs: H = 53.24, df = 3, P < 0.0001, η² = 0.9340, Kruskal-Wallis test; RFP Abs: H = 59.41, df = 3, P < 0.0001, η² = 0.8737, Kruskal-Wallis test). Data are represented as means ± SDs. AF: Alexa Fluor, CETE: center, Ctx: cerebral cortex, IR: immunoreactivity, NF: native fluorescence. PERIPH: periphery. Scale bar: 200 µm.

Article Snippet: Primary Abs used were; guinea pig (Gp) polyclonal anti-DsRed (red fluorescent protein) Ab (4.0 μg/mL, DsRed-GP-Af360, Frontier Institute, RRID: AB_2571648), rabbit (Rb) polyclonal anti-GFP Ab (10 μg/mL, A-11122, Thermo Fisher Scientific, RRID: AB_221569), rat (Rt) monoclonal anti-GFP Ab (1:1000, 04404-26, Nacalai Tesque, RRID: AB_2313652), chicken (Ch) polyclonal anti-Green Fluorescent Protein Ab (2.0 μg/mL, GFP-1020, Aves, RRID: AB_10000240), Alexa Fluor 647-conjugated anti-GFP nAb (1:500, Cheomotek, gb2AF647, RRID: AB_2827575), Alexa Fluor 647-conjugated goat (Gt) anti-HRP Ab (1:100, 123-605-021, Jackson Immuno Research, RRID: AB_2338967), Gt polyclonal anti-Iba1 Ab (1:1000, 011-27991, FUJIFILM Wako Pure Chemical Corporation, RRID:AB_2935833), mouse (Ms) monoclonal anti-RFP Ab (1:500, 409 011, Synaptic Systems, RRID: AB_2800533), and Gt polyclonal anti-tdTomato Ab (1:200, AB8181-200, SICGEN, RRID: AB_2722750).

Techniques: Infection, Fluorescence, Staining

Journal: Nature

Article Title: Inflammatory Memory Sensitizes Skin Epithelial Stem Cells to Tissue Damage

doi: 10.1038/nature24271

Figure Lengend Snippet: List of Flow Cytometry and Immunofluorescence Antibodies

Article Snippet: Rabbit Anti-Mouse RFP , MBL , Cat# PM005.

Techniques: Flow Cytometry, Immunofluorescence, Purification